Nutrient Agar is a general purpose, nutrient tool used for the cultivation of microbes supporting development of a wide selection of non-fastidious organisms. Nutrient agar is popular because it can flourish a selection of types of bacteria and fungi, and also contains many nutrients necessary for the bacterial growth.

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Composition that Nutrient Agar

0.5% Peptone

It is an enzymatic digest of pet protein. Peptone is the principal resource of organic nitrogen for the growing bacteria.

0.3% beef extract/yeast extract

It is the water-soluble building materials which aid in bacterial growth, such as vitamins, carbohydrates, necessary nitrogen compounds and salts.

1.5% agar

It is the solidifying agent.

0.5% NaCl

The presence of salt chloride in nutrient agar maintains a salt concentration in the medium that is similar to the cytoplasm the the microorganisms.

Distilled water

Water is crucial for the expansion of and also reproduction the micro-organisms and likewise provides the tool through which assorted nutrients have the right to be transported.

pH is changed to neutral (7.4) at 25 °C.

Preparation that Nutrient Agar1. Suspend 28 g that nutrient agar powder in 1 litre the distilled water.

2. Heat this mixture while stirring to fully dissolve every components.

3. Autoclave the dissolved mixture at 121 degrees Celsius because that 15 minutes.

4. Once the nutrient agar has been autoclaved, allow it come cool yet not solidify.

5. Pour nutrient agar into each plate and also leave plates on the sterile surface until the agar has actually solidified.

6. Replace the lid of every Petri dish and also store the bowl in a refrigerator.

Uses of nutrients Agar1. It is generally used because that isolation and also purification of cultures.

2. It can likewise be provided as a method for producing the bacter lawns needed for antibiotic sensitivity tests. In actuality, antibiotic sensitivity experimentation is commonly performed on media specially recipe for that purpose.

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Four nutrient agar plates growing swarms of common Gram an adverse bacteria.Source: Wikipedia
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